Catalog Number - 68112-05P

Cogent RP C12™ is a unique reversed-phase UHPLC stationary phase that utilizes a rigid adamantane-like carbon structure bonded to ultra-high purity silica. Fully double end capped, this phase provides excellent stability, rapid re-equilibration, and highly reproducible retention characteristics. The distinctive RP C12™ chemistry offers hydrophobic retention between traditional C8 and C18 phases while remaining compatible with 100% aqueous mobile phases without phase collapse, making it particularly valuable for high-throughput screening (HTS), gradient methods, and complex mixture separations.

68112-05P is a UHPLC column packed with 1.8 µm RP C12™ particles featuring a 100 Å pore size in a 4.6 mm ID x 50 mm format. The short 50 mm length is optimized for rapid analysis and high sample throughput, while the 1.8 µm particle technology provides exceptional efficiency, increased peak capacity, and superior chromatographic performance. This column is specifically designed for high-pressure UHPLC applications and delivers its full performance advantages when operated on UHPLC instrumentation capable of handling the elevated pressures generated by sub-2 µm particles. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on method conditions and instrument pressure limitations.

This column is ideal for high-throughput screening, pharmaceutical analysis, impurity profiling, and complex mixture separations. While it may be used on compatible HPLC systems, it is engineered primarily for UHPLC operation where the complete benefits of sub-2 µm particle technology—including superior efficiency, faster separations, higher peak capacity, and improved resolution—can be fully realized.

Key Benefits

• RP C12™ stationary phase

• Fully double end capped

• Unique adamantane-like bonded phase

• Ultra-high purity silica support

• Engineered for UHPLC applications

• 1.8 µm particle technology

• Exceptional column efficiency

• Rapid re-equilibration between runs

• Compatible with 100% aqueous mobile phases

• Increased peak capacity and throughput

Typical Applications

• UHPLC analysis

• High-throughput screening (HTS)

• Pharmaceutical analysis

• Impurity profiling

• Complex mixture separations

• Method development

• Gradient separations

• Quality control testing

• Environmental analysis

• High-speed reversed-phase chromatography

Specifications

• Product Type: UHPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane-Like Carbon Silane

• Endcapping: Fully Double End Capped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Specifically designed for UHPLC operation and high-pressure reversed-phase separations

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• May be used on compatible HPLC systems only if instrument pressure limits are not exceeded

• Sub-2 µm particles generate significantly higher backpressure than conventional HPLC columns

• Compatible with 100% aqueous mobile phases without loss of retention or phase collapse

• Rapid equilibration makes the phase especially useful for high-throughput screening applications

• Retention is typically stronger than C8 phases and lower than C18 phases for comparable hydrophobic compounds

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

• Short column length provides rapid analysis and increased laboratory throughput

  ---